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S100A9 antibody

Anti-S100A9 antibody used in IHC (Paraffin sections) (IHC-P). GTX129575
Anti-S100A9 antibody used in Western Blot (WB). GTX129575
Anti-S100A9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129575
Anti-S100A9 antibody used in IHC (Paraffin sections) (IHC-P). GTX129575

Cat No. GTX129575

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human S100A9 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

S100 calcium binding protein A9 , 60B8AG , CAGB , CFAG , CGLB , L1AG , LIAG , MAC387 , MIF , MRP14 , NIF , P14

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-S100A9 antibody used in IHC (Paraffin sections) (IHC-P). GTX129575

GTX129575 IHC-P Image

S100A9 antibody detects S100A9 protein at secreted on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
S100A9 antibody (GTX129575) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-S100A9 antibody used in Western Blot (WB). GTX129575

GTX129575 WB Image

S100A9 antibody detects S100A9 protein by western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
15 % SDS-PAGE
S100A9 antibody (GTX129575) dilution: 1:1000

Anti-S100A9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129575

GTX129575 ICC/IF Image

S100A9 antibody detects S100A9 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: S100A9 protein stained by S100A9 antibody (GTX129575) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-S100A9 antibody used in IHC (Paraffin sections) (IHC-P). GTX129575

GTX129575 IHC-P Image

S100A9 antibody detects S100A9 protein at secreted on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
S100A9 antibody (GTX129575) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169