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S100A9 antibody

Anti-S100A9 antibody used in IHC (Paraffin sections) (IHC-P). GTX129575
Anti-S100A9 antibody used in IHC (Paraffin sections) (IHC-P). GTX129575
Anti-S100A9 antibody used in Western Blot (WB). GTX129575
Anti-S100A9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129575

Cat. No. GTX129575

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all S100A9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human S100A9 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886038

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

S100 calcium binding protein A9 , 60B8AG , CAGB , CFAG , CGLB , L1AG , LIAG , MAC387 , MIF , MRP14 , NIF , P14

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-S100A9 antibody used in IHC (Paraffin sections) (IHC-P). GTX129575

GTX129575 IHC-P Image

S100A9 antibody detects S100A9 protein at secreted on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
S100A9 antibody (GTX129575) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-S100A9 antibody used in IHC (Paraffin sections) (IHC-P). GTX129575

GTX129575 IHC-P Image

S100A9 antibody detects S100A9 protein at secreted on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
S100A9 antibody (GTX129575) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-S100A9 antibody used in Western Blot (WB). GTX129575

GTX129575 WB Image

S100A9 antibody detects S100A9 protein by western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
15 % SDS-PAGE
S100A9 antibody (GTX129575) dilution: 1:1000

Anti-S100A9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129575

GTX129575 ICC/IF Image

S100A9 antibody detects S100A9 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: S100A9 protein stained by S100A9 antibody (GTX129575) diluted at 1:250.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Mori S et al. J Virol 2023; 97 (8) : e0081523 Nuclear proinflammatory cytokine S100A9 enhances expression of human papillomavirus oncogenes via transcription factor TEAD1.
WC Liao et al. BMC Cancer 2023; 23 : 513 S100A8, S100A9 and S100A8/A9 heterodimer as novel cachexigenic factors for pancreatic cancer-induced cachexia
Application : WB, IHC-P
Reactivity : Human
Seto S et al. Front Microbiol 2019; 10 : 3081 Proteomic Profiling Reveals the Architecture of Granulomatous Lesions Caused by Tuberculosis and Mycobacterium avium Complex Lung Disease.
Application : IHC-P
Reactivity : Human
Kato R et al. Drug Metab Dispos 2019; The 2-Hydroxyiminostilbene Metabolite of Carbamazepine or the Supernatant from Incubation of Hepatocytes with Carbamazepine Activates Inflammasomes; Implications for Carbamazepine-induced Hypersensitivity Reactions.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169