S1PR2 antibody [HL3765]

S1PR2 antibody [HL3765](GTX641964) detects S1PR2 protein by flow cytometry analysis.
Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer.
The cells were following stained with S1PR2 antibody [HL3765](GTX641964) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) at 4ºC for 1 hour.

Unboiled various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with S1PR2 antibody [HL3765] (GTX641964) diluted at 1:2400. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

Unboiled Raji whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with S1PR2 antibody [HL3765] (GTX641964) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with S1PR2 antibody [HL3765] (GTX641964) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

Boiled and unboiled HCT116 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with S1PR2 antibody [HL3765] (GTX641964) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.(WCE: whole cell extract; ME: membrane extract)

Unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with S1PR2 antibody [HL3765] (GTX641964) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.