Unboiled mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with S1PR5 / EDG8 antibody [HL4092] (GTX642531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with S1PR5 / EDG8 antibody [HL4092] (GTX642531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.