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SA1 antibody [GT1515]

KO/KD
Anti-SA1 antibody [GT1515] used in Western Blot (WB). GTX632010
Anti-SA1 antibody [GT1515] used in Western Blot (WB). GTX632010
Anti-SA1 antibody [GT1515] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632010
Anti-SA1 antibody [GT1515] used in Immunoprecipitation (IP). GTX632010
Anti-SA1 antibody [GT1515] used in Western Blot (WB). GTX632010

Cat. No. GTX632010

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1515

Isotype

 IgG2b

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

144 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SA1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888274

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stromal antigen 1 , MRD47 , SA1 , SCC3A

Cellular Localization

Nucleus

Background

This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SA1 antibody [GT1515] used in Western Blot (WB). GTX632010

GTX632010 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (GTX632010) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SA1 antibody [GT1515] used in Western Blot (WB). GTX632010

GTX632010 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (GTX632010) diluted at 1:500.

Anti-SA1 antibody [GT1515] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632010

GTX632010 ICC/IF Image

SA1 antibody [GT1515] detects SA1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SA1 protein stained by SA1 antibody [GT1515] (GTX632010) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SA1 antibody [GT1515] used in Immunoprecipitation (IP). GTX632010

GTX632010 IP Image

Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 μg of SA1 antibody [GT1515] (GTX632010).
Western blot analysis was performed using SA1 antibody [GT1515] (GTX632010).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-SA1 antibody [GT1515] used in Western Blot (WB). GTX632010

GTX632010 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (GTX632010) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations