Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (GTX632010) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 μg of SA1 antibody [GT1515] (GTX632010).
Western blot analysis was performed using SA1 antibody [GT1515] (GTX632010).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

SA1 antibody [GT1515] detects SA1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SA1 protein stained by SA1 antibody [GT1515] (GTX632010) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (GTX632010) diluted at 1:500.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT1515] (GTX632010) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.