Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT8810] (GTX631989) diluted at 1:500.

Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 μg of SA1 antibody [GT8810] (GTX631989).
Western blot analysis was performed using SA1 antibody [GT8810] (GTX631989) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

SA1 antibody [GT8810] detects SA1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SA1 protein stained by SA1 antibody [GT8810] (GTX631989) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody (GTX631989) diluted at 1:1000.