APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Mouse, Rat, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SA1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888269
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
stromal antigen 1 , MRD47 , SA1 , SCC3A
Cellular Localization
Nucleus
Background
This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-SA1 antibody [GT8810] used in Western Blot (WB). GTX631989](/templates/default/js/lightbox/images/loading.gif "Anti-SA1 antibody [GT8810] used in Western Blot (WB). GTX631989")
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GTX631989 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody [GT8810] (GTX631989) diluted at 1:500.
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GTX631989 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody (GTX631989) diluted at 1:1000.
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GTX631989 ICC/IF Image
SA1 antibody [GT8810] detects SA1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SA1 protein stained by SA1 antibody [GT8810] (GTX631989) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX631989 IP Image
Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 μg of SA1 antibody [GT8810] (GTX631989).
Western blot analysis was performed using SA1 antibody [GT8810] (GTX631989) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
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REFERENCE
There are currently no references for SA1 antibody [GT8810] (GTX631989). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SA1 antibody [GT8810] (GTX631989). Be the first to share your experience with this product.
![Anti-SA1 antibody [GT8810] used in Western Blot (WB). GTX631989](/upload/website/prouct_img/small/GTX631989/GTX631989_41891_20161013_WB_shRNA_watermark_w_23061202_397.webp "Anti-SA1 antibody [GT8810] used in Western Blot (WB). GTX631989")
![Anti-SA1 antibody [GT8810] used in Western Blot (WB). GTX631989](/upload/website/prouct_img/small/GTX631989/GTX631989_41891_20160517_WB_w_23061202_409.webp "Anti-SA1 antibody [GT8810] used in Western Blot (WB). GTX631989")
![Anti-SA1 antibody [GT8810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631989](/upload/website/prouct_img/small/GTX631989/GTX631989_41891_20141112_IFA_w_23061202_882.webp "Anti-SA1 antibody [GT8810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631989")
![Anti-SA1 antibody [GT8810] used in Immunoprecipitation (IP). GTX631989](/upload/website/prouct_img/small/GTX631989/GTX631989_41891_20160517_IP_w_23061202_721.webp "Anti-SA1 antibody [GT8810] used in Immunoprecipitation (IP). GTX631989")
![Anti-SA1 antibody [GT8810] used in Western Blot (WB). GTX631989](/upload/website/prouct_img/normal/GTX631989/GTX631989_41891_20161013_WB_shRNA_watermark_w_23061202_397.webp "Anti-SA1 antibody [GT8810] used in Western Blot (WB). GTX631989")
![Anti-SA1 antibody [GT8810] used in Western Blot (WB). GTX631989](/upload/website/prouct_img/normal/GTX631989/GTX631989_41891_20160517_WB_w_23061202_409.webp "Anti-SA1 antibody [GT8810] used in Western Blot (WB). GTX631989")
![Anti-SA1 antibody [GT8810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631989](/upload/website/prouct_img/normal/GTX631989/GTX631989_41891_20141112_IFA_w_23061202_882.webp "Anti-SA1 antibody [GT8810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631989")
![Anti-SA1 antibody [GT8810] used in Immunoprecipitation (IP). GTX631989](/upload/website/prouct_img/normal/GTX631989/GTX631989_41891_20160517_IP_w_23061202_721.webp "Anti-SA1 antibody [GT8810] used in Immunoprecipitation (IP). GTX631989")