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SA1 antibody

KO/KD
Anti-SA1 antibody used in Western Blot (WB). GTX130364
Anti-SA1 antibody used in Western Blot (WB). GTX130364
Anti-SA1 antibody used in Immunoprecipitation (IP). GTX130364
Anti-SA1 antibody used in Western Blot (WB). GTX130364

Cat. No. GTX130364

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

144 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886246

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stromal antigen 1 , MRD47 , SA1 , SCC3A

Cellular Localization

Nucleus

Background

This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SA1 antibody used in Western Blot (WB). GTX130364

GTX130364 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody (GTX130364) diluted at 1:3000.

Anti-SA1 antibody used in Western Blot (WB). GTX130364

GTX130364 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody (GTX130364) diluted at 1:1000.

Anti-SA1 antibody used in Immunoprecipitation (IP). GTX130364

GTX130364 IP Image

Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 μg of SA1 antibody (GTX130364).
Western blot analysis was performed using SA1 antibody (GTX130364) diluted at 1:1000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SA1 antibody used in Western Blot (WB). GTX130364

GTX130364 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody (GTX130364) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169