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SA2 antibody

Anti-SA2 antibody used in Western Blot (WB). GTX129876
Anti-SA2 antibody used in Western Blot (WB). GTX129876
Anti-SA2 antibody used in Immunoprecipitation (IP). GTX129876
Anti-SA2 antibody used in Western Blot (WB). GTX129876
Anti-SA2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129876
Anti-SA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129876

Cat No. GTX129876

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rat, Rabbit, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

stromal antigen 2 , SA-2 , SA2 , SCC3B , bA517O1.1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

Database

Research Area

DATA IMAGES

Anti-SA2 antibody used in Western Blot (WB). GTX129876

GTX129876 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX129876) diluted at 1:2000.

Anti-SA2 antibody used in Western Blot (WB). GTX129876

GTX129876 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX129876) diluted at 1:1000.

Anti-SA2 antibody used in Immunoprecipitation (IP). GTX129876

GTX129876 IP Image

Immunoprecipitation of SA2 protein from 293T whole cell extracts using 5 μg of SA2 antibody (GTX129876).
Western blot analysis was performed using SA2 antibody (GTX129876).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SA2 antibody used in Western Blot (WB). GTX129876

GTX129876 WB Image

Various whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX129876) diluted at 1:1000.

Anti-SA2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129876

GTX129876 ICC/IF Image

SA2 antibody detects SA2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SA2 stained by SA2 antibody (GTX129876) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10μm.

Anti-SA2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129876

GTX129876 IHC-P Image

SA2 antibody detects SA2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SA2 stained by SA2 antibody (GTX129876) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169