Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX130299) diluted at 1:6000.

SA2 antibody detects SA2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SA2 protein stained by SA2 antibody (GTX130299) diluted at 1:500.
Red: Phalloidin, a F-actin marker, diluted at 1:200.

Immunoprecipitation of SA2 protein from HeLa whole cell extracts using 5 μg of SA2 antibody (GTX130299).
Western blot analysis was performed using SA2 antibody (GTX130299).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX130299) diluted at 1:1000.