Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX130304) diluted at 1:2000.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX130304) diluted at 1:1000.

Immunoprecipitation of SA2 protein from 293T whole cell extracts using 5 μg of SA2 antibody (GTX130304).
Western blot analysis was performed using SA2 antibody (GTX130304) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

SA2 antibody detects SA2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SA2 stained by SA2 antibody (GTX130304) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min