GeneTex
United States (US)

SA3 antibody

STAG3-antibody-GTX87952-WB-1_18122102_632.jpg

Cat No. GTX87952

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:10000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

139 kDa. ( Note )

Specificity/Sensitivity

STAG3 Antibody detects endogenous levels of total STAG3 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acids 1161 and 1210 of Human SA3.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Stromal Antigen 3,Stag3

Cellular Localization

Associates with chromatin. In prophase I stage of meiosis, it is found along the axial elements of synaptonemal complexes. In late-pachytene-diplotene, the bulk of protein dissociates from the chromosome arms probably because of phosphorylation by PLK

Background

Meiosis specific component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I.

Database

Research Area

DATA IMAGES
STAG3-antibody-GTX87952-WB-1_18122102_632.jpg

GTX87952 WB Image

Western blot analysis of extracts from Jurkat cells, using STAG3 Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339