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SAE1 antibody, C-term

Anti-SAE1 antibody, C-term used in Western Blot (WB). GTX25645

Cat. No. GTX25645

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all SAE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1μg/ml
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX25645-PEP).

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence MKGNGIVECLGPK, from the C Terminus of the protein sequence according to NP_005491.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SUMO1 activating enzyme subunit 1 , AOS1 , HSPC140 , SUA1 , UBLE1A

Cellular Localization

Nucleus

Background

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-SAE1 antibody, C-term used in Western Blot (WB). GTX25645

GTX25645 WB Image

WB analysis of Jurkat lysate using GTX25645 SAE1 antibody, C-term.
Dilution : 1μg/ml
Loading : 30μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399