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SAE1 antibody

KO/KD
Anti-SAE1 antibody used in Western Blot (WB). GTX113602
Anti-SAE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113602
Anti-SAE1 antibody used in Immunoprecipitation (IP). GTX113602
Anti-SAE1 antibody used in Western Blot (WB). GTX113602
Anti-SAE1 antibody used in Western Blot (WB). GTX113602
Anti-SAE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113602
Anti-SAE1 antibody used in Western Blot (WB). GTX113602

Cat. No. GTX113602

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SAE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

Mouse brain , Rat2 , A431 , H1299

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SAE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037209

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SUMO1 activating enzyme subunit 1 , AOS1 , HSPC140 , SUA1 , UBLE1A

Cellular Localization

Nucleus

Background

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SAE1 antibody used in Western Blot (WB). GTX113602

GTX113602 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
10% SDS PAGE
GTX113602 diluted at 1:1000

Anti-SAE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113602

GTX113602 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using SAE1(GTX113602) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SAE1 antibody used in Immunoprecipitation (IP). GTX113602

GTX113602 IP Image

Immunoprecipitation of SAE1 protein from HepG2 whole cell extracts using 5 μg of SAE1 antibody (GTX113602).
Western blot analysis was performed using SAE1 antibody (GTX113602).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SAE1 antibody used in Western Blot (WB). GTX113602

GTX113602 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX113602 diluted at 1:1000

Anti-SAE1 antibody used in Western Blot (WB). GTX113602

GTX113602 WB Image

SAE1 antibody detects SAE1 protein by Western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
SAE1 antibody (GTX113602) dilution: 1:1000

Anti-SAE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113602

GTX113602 ICC/IF Image

SAE1 antibody detects SAE1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SAE1 protein stained by SAE1 antibody (GTX113602) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-SAE1 antibody used in Western Blot (WB). GTX113602

GTX113602 WB Image

KO/KD

The data was published in the journal Genes (Basel) in 2014.PMID: 25333844

REFERENCE

Application Reference

Zhang H et al. Genes (Basel) 2014; 5 (4) : 982-1000 Sumoylation is Required for the Cytoplasmic Accumulation of a Subset of mRNAs.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169