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SAE1 antibody

Anti-SAE1 antibody used in Western Blot (WB). GTX31803

Cat No. GTX31803

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

SK-N-SH Cell Lysate(GTX23956)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SAE1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human SAE1.The immunogen is located within amino acids 240 - 290 of SAE1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SUMO1 activating enzyme subunit 1 , AOS1 , HSPC140 , SUA1 , UBLE1A

Cellular Localization

Nucleus

Background

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-SAE1 antibody used in Western Blot (WB). GTX31803

GTX31803 WB Image

WB analysis of SK-N-SH lysate in (A) the absence and (B) the presence of blocking peptide using GTX31803 SAE1 antibody.
Working concentration : 0.5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf