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SAFB antibody [2E8-E2-G6]

Anti-SAFB antibody [2E8-E2-G6] used in Western Blot (WB). GTX49227
Anti-SAFB antibody [2E8-E2-G6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49227

Cat No. GTX49227

Host

Mouse

Clonality

Monoclonal

Clone Name

2E8-E2-G6

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human SAFB protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Scaffold Attachment Factor B , Hap , Het , Sab-B1 , Saf-B , Saf-B1 , Safb1 , Safb

Cellular Localization

Nucleus

Background

This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a ´transcriptosome complex´ in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.

Database

Research Area

DATA IMAGES

Anti-SAFB antibody [2E8-E2-G6] used in Western Blot (WB). GTX49227

GTX49227 WB Image

WB analysis of indicated lysates using SAFB1 antibody at a dilution of 1:4,000.

Anti-SAFB antibody [2E8-E2-G6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49227

GTX49227 ICC/IF Image

ICC/IF analysis of HeLa cells using SAFB1 antibody at a dilution of 1:300.

REFERENCE

There are currently no references for SAFB antibody [2E8-E2-G6] (GTX49227). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SAFB antibody [2E8-E2-G6] (GTX49227). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf