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SAFB antibody

Anti-SAFB antibody used in Western Blot (WB). GTX101450
Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450
Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450
Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450
Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450
Anti-SAFB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101450

Cat. No. GTX101450

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SAFB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SAFB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037161

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

scaffold attachment factor B , HAP , HET , SAB-B1 , SAF-B , SAF-B1 , SAFB1

Cellular Localization

Nucleus

Background

This gene encodes a DNA-binding protein that has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. This encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of the heat shock protein 27 transcription and also can act as an estrogen receptor corepressor. This gene is a candidate gene for breast tumorigenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SAFB antibody used in Western Blot (WB). GTX101450

GTX101450 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Scaffold attachment factor B1 antibody (GTX101450) diluted at 1:1000.

Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450

GTX101450 IHC-P Image

Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Scaffold attachment factor B1 antibody (GTX101450) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450

GTX101450 IHC-P Image

Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Scaffold attachment factor B1 antibody (GTX101450) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450

GTX101450 IHC-P Image

Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Scaffold attachment factor B1 antibody (GTX101450) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450

GTX101450 IHC-P Image

Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Scaffold attachment factor B1 antibody (GTX101450) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SAFB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101450

GTX101450 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Scaffold attachment factor B1(GTX101450) antibody at 1:100 dilution.

REFERENCE

Application Reference

Luo S. et al. Basic Res Cardiol. 2021; 116 (1) : 15 SAIL: a new conserved anti-fibrotic lncRNA in the heart.
Application : WB
Reactivity : Mouse
Takeuchi A et al. J Autoimmun 2016; (Epub) Autoantibody to scaffold attachment factor B (SAFB): A novel connective tissue disease-related autoantibody associated with interstitial lung disease.
Application : WB
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB
Reactivity : Human
Hong E et al. Biomed Res Int 2015; 2015 : 395816 Unravelling the RNA-Binding Properties of SAFB Proteins in Breast Cancer Cells.
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169