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SAFB antibody

Anti-SAFB antibody used in Western Blot (WB). GTX101450
Anti-SAFB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101450
Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450
Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450
Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450
Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450

Cat No. GTX101450

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SAFB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

scaffold attachment factor B,HAP,HET,SAB-B1,SAF-B,SAF-B1,SAFB1

Cellular Localization

Nucleus

Background

This gene encodes a DNA-binding protein that has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. This encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of the heat shock protein 27 transcription and also can act as an estrogen receptor corepressor. This gene is a candidate gene for breast tumorigenesis. [provided by RefSeq]

Database

DATA IMAGES
Anti-SAFB antibody used in Western Blot (WB). GTX101450

GTX101450 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Scaffold attachment factor B1 antibody (GTX101450) diluted at 1:1000.

Anti-SAFB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101450

GTX101450 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Scaffold attachment factor B1(GTX101450) antibody at 1:100 dilution.

Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450

GTX101450 IHC-P Image

Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Scaffold attachment factor B1 antibody (GTX101450) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450

GTX101450 IHC-P Image

Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Scaffold attachment factor B1 antibody (GTX101450) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450

GTX101450 IHC-P Image

Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Scaffold attachment factor B1 antibody (GTX101450) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX101450

GTX101450 IHC-P Image

Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Scaffold attachment factor B1 antibody (GTX101450) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169