GeneTex
United States (US)

SAFB2 antibody [N2C1], Internal

GTX115272_40282_IFA_18121410_646.jpg
GTX115272_40282_IHC_18121410_883.jpg
GTX115272_WB_18121410_931.jpg
GTX115272_40282_20150624_IHC-P_M_18121410_625.jpg
GTX115272_40282_20150624_IHC-P_M_2_18121410_731.jpg

Cat No. GTX115272

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

Positive Control

293T , A431 , Jurkat

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Scaffold attachment factor B2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Scaffold Attachment Factor B2,Safb2

Background

Binds to scaffold/matrix attachment region (S/MAR) DNA. Can function as an estrogen receptor corepressor and can also inhibit cell proliferation.

Database

DATA IMAGES
GTX115272_40282_IFA_18121410_646.jpg

GTX115272 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Scaffold attachment factor B2(GTX115272) antibody at 1:200 dilution.

GTX115272_40282_IHC_18121410_883.jpg

GTX115272 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Scaffold attachment factor B2(GTX115272) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115272_WB_18121410_931.jpg

GTX115272 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX115272 diluted at 1:1000

GTX115272_40282_20150624_IHC-P_M_18121410_625.jpg

GTX115272 IHC-P Image

SAFB2 antibody [N2C1], Internal detects SAFB2 protein at nucleus in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SAFB2 antibody [N2C1], Internal (GTX115272) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115272_40282_20150624_IHC-P_M_2_18121410_731.jpg

GTX115272 IHC-P Image

SAFB2 antibody [N2C1], Internal detects SAFB2 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SAFB2 antibody [N2C1], Internal (GTX115272) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169