SALL4 antibody [C1C2], Internal

Cat No. GTX109983

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Pig
Package	100 μl ($329), 25 μl ($169)

See all SALL4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

A431

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SALL4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951803

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

spalt like transcription factor 4 , DRRS , HSAL4 , ZNF797

Cellular Localization

Nucleus

Background

The protein encoded by this gene may be a zinc finger transcription factor. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS). [provided by RefSeq]

Database

Gene ID: 57167 SALL4
UniProt: Q9UJQ4 SALL4

Research Area

DATA IMAGES

GTX109983 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SALL4(GTX109983) antibody at 1:400 dilution.

GTX109983 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SALL4(GTX109983) antibody at 1:500 dilution.

GTX109983 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SALL4(GTX109983) antibody at 1:400 dilution.

GTX109983 WB Image

SALL4 antibody [C1C2], Internal detects SALL4 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and blotted with SALL4 antibody [C1C2], Internal (GTX109983) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109983 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft , using SALL4(GTX109983) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min