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SALL4 antibody [C1C2], Internal

Anti-SALL4 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109983
Anti-SALL4 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109983
Anti-SALL4 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109983
Anti-SALL4 antibody [C1C2], Internal used in Western Blot (WB). GTX109983
Anti-SALL4 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109983

Cat No. GTX109983

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Pig
Package
100 μl ($379),
25 μl ($169)
See all SALL4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

A431

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SALL4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951803

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

spalt like transcription factor 4 , DRRS , HSAL4 , ZNF797

Cellular Localization

Nucleus

Background

The protein encoded by this gene may be a zinc finger transcription factor. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SALL4 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109983

GTX109983 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SALL4(GTX109983) antibody at 1:400 dilution.

Anti-SALL4 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109983

GTX109983 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SALL4(GTX109983) antibody at 1:500 dilution.

Anti-SALL4 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109983

GTX109983 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SALL4(GTX109983) antibody at 1:400 dilution.

Anti-SALL4 antibody [C1C2], Internal used in Western Blot (WB). GTX109983

GTX109983 WB Image

SALL4 antibody [C1C2], Internal detects SALL4 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and blotted with SALL4 antibody [C1C2], Internal (GTX109983) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SALL4 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109983

GTX109983 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft , using SALL4(GTX109983) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Xu J et al. Stem Cell Res Ther 2019; 10 (1) : 193 Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system.
Yang F et al. J Cell Physiol 2017; ESRRB plays a crucial role in the promotion of porcine cell reprogramming.
Application : ICC/IF
Reactivity : Human
Gautam AK et al. Oncol Lett 2015; 10 (6) : 3629-3634 Expression and clinical significance of SALL4 and LGR5 in patients with lung cancer.
Liu S et al. Dev Dyn 2015; SOX2 is the faithful marker for pluripotency in pig: Evidence from embryonic studies.
Application : ICC/IF
Reactivity : Pig
Chen Y et al. Hepatology 2012; 55 (2) : 563-74 Mature hepatocytes exhibit unexpected plasticity by direct dedifferentiation into liver progenitor cells in culture.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169