SALL4 antibody [C1C2], Internal

Cat. No. GTX109983

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Pig
Package	100 μl ($399), 25 μl ($169)

See all SALL4 products

View product citations for antibody GTX109983 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

A431

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SALL4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951803

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

spalt like transcription factor 4 , DRRS , HSAL4 , ZNF797

Cellular Localization

Nucleus

Background

The protein encoded by this gene may be a zinc finger transcription factor. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS). [provided by RefSeq]

Database

Gene ID: 57167 SALL4
UniProt: Q9UJQ4 SALL4

Research Area

DATA IMAGES

GTX109983 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft , using SALL4(GTX109983) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109983 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SALL4(GTX109983) antibody at 1:500 dilution.

GTX109983 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SALL4(GTX109983) antibody at 1:400 dilution.

GTX109983 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SALL4(GTX109983) antibody at 1:400 dilution.

GTX109983 WB Image

SALL4 antibody [C1C2], Internal detects SALL4 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and blotted with SALL4 antibody [C1C2], Internal (GTX109983) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.