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SALL4 antibody [C1C2], Internal

Anti-SALL4 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109983
Anti-SALL4 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109983
Anti-SALL4 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109983
Anti-SALL4 antibody [C1C2], Internal used in Western Blot (WB). GTX109983
Anti-SALL4 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109983

Cat No. GTX109983

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Pig
Package
100 μl ($329),
25 μl ($169)
See all SALL4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

A431

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SALL4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

spalt like transcription factor 4 , DRRS , HSAL4 , ZNF797

Cellular Localization

Nucleus

Background

The protein encoded by this gene may be a zinc finger transcription factor. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SALL4 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109983

GTX109983 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SALL4(GTX109983) antibody at 1:400 dilution.

Anti-SALL4 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109983

GTX109983 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SALL4(GTX109983) antibody at 1:500 dilution.

Anti-SALL4 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109983

GTX109983 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SALL4(GTX109983) antibody at 1:400 dilution.

Anti-SALL4 antibody [C1C2], Internal used in Western Blot (WB). GTX109983

GTX109983 WB Image

SALL4 antibody [C1C2], Internal detects SALL4 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and blotted with SALL4 antibody [C1C2], Internal (GTX109983) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SALL4 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX109983

GTX109983 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft , using SALL4(GTX109983) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Xu J et al. Stem Cell Res Ther 2019; 10 (1) : 193 Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system.
Yang F et al. J Cell Physiol 2017; ESRRB plays a crucial role in the promotion of porcine cell reprogramming.
Application : ICC/IF
Reactivity : Human
Gautam AK et al. Oncol Lett 2015; 10 (6) : 3629-3634 Expression and clinical significance of SALL4 and LGR5 in patients with lung cancer.
Liu S et al. Dev Dyn 2015; SOX2 is the faithful marker for pluripotency in pig: Evidence from embryonic studies.
Application : ICC/IF
Reactivity : Pig
Chen Y et al. Hepatology 2012; 55 (2) : 563-74 Mature hepatocytes exhibit unexpected plasticity by direct dedifferentiation into liver progenitor cells in culture.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169