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SAM68 antibody [JE47-15]

Recombinant antibody
Anti-SAM68 antibody [JE47-15] used in Western Blot (WB). GTX01166
Anti-SAM68 antibody [JE47-15] used in Western Blot (WB). GTX01166
Anti-SAM68 antibody [JE47-15] used in Western Blot (WB). GTX01166
Anti-SAM68 antibody [JE47-15] used in Western Blot (WB). GTX01166
Anti-SAM68 antibody [JE47-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01166
Anti-SAM68 antibody [JE47-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01166
Anti-SAM68 antibody [JE47-15] used in IHC (Paraffin sections) (IHC-P). GTX01166
Anti-SAM68 antibody [JE47-15] used in IHC (Paraffin sections) (IHC-P). GTX01166
Anti-SAM68 antibody [JE47-15] used in IHC (Paraffin sections) (IHC-P). GTX01166

Cat No. GTX01166

Host

Rabbit

Clonality

Monoclonal

Clone Name

JE47-15

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ICC/IF 1:50
IHC-P 1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Observed MW

68 kDa. The observed M.W. is based on the following publication. PMID: 27415018

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

KH RNA binding domain containing, signal transduction associated 1 , KHDRBS1 , Sam68 , p62 , p68 , SAM68

Cellular Localization

Nucleus,Membrane

Background

This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3'-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

Database

Research Area

DATA IMAGES

Anti-SAM68 antibody [JE47-15] used in Western Blot (WB). GTX01166

GTX01166 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SAM68 antibody [JE47-15] (GTX01166) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SAM68 antibody [JE47-15] used in Western Blot (WB). GTX01166

GTX01166 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SAM68 antibody [JE47-15] (GTX01166) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SAM68 antibody [JE47-15] used in Western Blot (WB). GTX01166

GTX01166 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SAM68 antibody [JE47-15] (GTX01166) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SAM68 antibody [JE47-15] used in Western Blot (WB). GTX01166

GTX01166 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SAM68 antibody [JE47-15] (GTX01166) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 27415018

Anti-SAM68 antibody [JE47-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01166

GTX01166 ICC/IF Image

ICC/IF analysis of PFA-fixed F9 cells using GTX01166 SAM68 antibody [JE47-15].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in PBS
Dilution : 1:50

Anti-SAM68 antibody [JE47-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01166

GTX01166 ICC/IF Image

ICC/IF analysis of PFA-fixed A431 cells using GTX01166 SAM68 antibody [JE47-15].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in PBS
Dilution : 1:50

Anti-SAM68 antibody [JE47-15] used in IHC (Paraffin sections) (IHC-P). GTX01166

GTX01166 IHC-P Image

IHC-P analysis of human colon cancer tissue section using GTX01166 SAM68 antibody [JE47-15].
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes.
Dilution : 1:1000

Anti-SAM68 antibody [JE47-15] used in IHC (Paraffin sections) (IHC-P). GTX01166

GTX01166 IHC-P Image

IHC-P analysis of human stomach cancer tissue section using GTX01166 SAM68 antibody [JE47-15].
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes.
Dilution : 1:1000

Anti-SAM68 antibody [JE47-15] used in IHC (Paraffin sections) (IHC-P). GTX01166

GTX01166 IHC-P Image

IHC-P analysis of human liver cancer tissue section using GTX01166 SAM68 antibody [JE47-15].
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes.
Dilution : 1:1000

REFERENCE

There are currently no references for SAM68 antibody [JE47-15] (GTX01166). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SAM68 antibody [JE47-15] (GTX01166). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf