GeneTex
United States (US)

SAM68 antibody [N2C3]

GTX115462_40646_20190118_IHC-P_M_19022001_482.jpg
GTX115462_40646_IHC_R_18121410_268.jpg
GTX115462_40646_WB_R_18121410_997.jpg
GTX115462_40646_WB_18121410_853.jpg
GTX115462_40646_20181115_ICC_IF_R_19011519_772.jpg

Cat No. GTX115462

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Jurakt , PC-12 , Rat2

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SAM68. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Kh Rna Binding Domain Containing, Signal Transduction Associated 1,Sam68,P62,P68,Khdrbs1

Cellular Localization

Nucleus , Membrane

Database

Research Area

DATA IMAGES
GTX115462_40646_20190118_IHC-P_M_19022001_482.jpg

GTX115462 IHC-P Image

SAM68 antibody [N2C3] detects SAM68 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SAM68 stained by SAM68 antibody [N2C3] (GTX115462) diluted at 1:500.
Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

GTX115462_40646_IHC_R_18121410_268.jpg

GTX115462 IHC-P Image

SAM68 antibody [N2C3] detects SAM68 protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
SAM68 antibody [N2C3] (GTX115462) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115462_40646_WB_R_18121410_997.jpg

GTX115462 WB Image

SAM68 antibody [N2C3] detects SAM68 protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
B. 30 μg Rat2 whole cell extract
7.5 % SDS-PAGE
SAM68 antibody [N2C3] (GTX115462) dilution: 1:3000

GTX115462_40646_WB_18121410_853.jpg

GTX115462 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
10% SDS PAGE
GTX115462 diluted at 1:1000

GTX115462_40646_20181115_ICC_IF_R_19011519_772.jpg

GTX115462 ICC/IF Image

SAM68 antibody [N2C3] detects SAM68 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SAM68 stained by SAM68 antibody [N2C3] (GTX115462) diluted at 1:2000.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf