Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SAM68 antibody [N2C3] (GTX115462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SAM68 antibody [N2C3] detects SAM68 protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
SAM68 antibody [N2C3] (GTX115462) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Jurkat
10% SDS PAGE
GTX115462 diluted at 1:1000

SAM68 antibody [N2C3] detects SAM68 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SAM68 stained by SAM68 antibody [N2C3] (GTX115462) diluted at 1:500.
Antigen Retrieval: Trilogy™M (EDTA based) buffer, 15min

SAM68 antibody [N2C3] detects SAM68 protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
B. 30 μg Rat2 whole cell extract
7.5 % SDS-PAGE
SAM68 antibody [N2C3] (GTX115462) dilution: 1:3000

SAM68 antibody [N2C3] detects SAM68 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SAM68 stained by SAM68 antibody [N2C3] (GTX115462) diluted at 1:2000.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).