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SAMHD1 antibody [1F6]

Overexpression
Anti-SAMHD1 antibody [1F6] used in Western Blot (WB). GTX83687
Anti-SAMHD1 antibody [1F6] used in Western Blot (WB). GTX83687
Anti-SAMHD1 antibody [1F6] used in Flow cytometry (FACS). GTX83687
Anti-SAMHD1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83687
Anti-SAMHD1 antibody [1F6] used in Flow cytometry (FACS). GTX83687
Anti-SAMHD1 antibody [1F6] used in Western Blot (WB). GTX83687
Anti-SAMHD1 antibody [1F6] used in Western Blot (WB). GTX83687
Anti-SAMHD1 antibody [1F6] used in Western Blot (WB). GTX83687

Cat. No. GTX83687

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1F6

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($519)
See all SAMHD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SAMHD1 (NP_056289) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10728075

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1 , CHBL2 , DCIP , HDDC1 , MOP-5 , SBBI88 , hSAMHD1

Cellular Localization

Nucleus

Background

Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.

Database

Research Area

DATA IMAGES

Anti-SAMHD1 antibody [1F6] used in Western Blot (WB). GTX83687

GTX83687 WB Image

Overexpression

WB analysis of HEK293T cells transfected with SAMHD1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83687 SAMHD1 antibody [1F6].
Loading : 5 ug per lane

Anti-SAMHD1 antibody [1F6] used in Western Blot (WB). GTX83687

GTX83687 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83687 SAMHD1 antibody [1F6].
Loading : 10 ug per lane
Dilution : 1:200

Anti-SAMHD1 antibody [1F6] used in Flow cytometry (FACS). GTX83687

GTX83687 FACS Image

FACS analysis of HeLa cells using GTX83687 SAMHD1 antibody [1F6].
Red : Primary antibody
Blue : Negative control antibody

Anti-SAMHD1 antibody [1F6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83687

GTX83687 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SAMHD1 plasmid using GTX83687 SAMHD1 antibody [1F6].

Anti-SAMHD1 antibody [1F6] used in Flow cytometry (FACS). GTX83687

GTX83687 FACS Image

FACS analysis of HEK293T cells transfected with either SAMHD1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83687 SAMHD1 antibody [1F6].

Anti-SAMHD1 antibody [1F6] used in Western Blot (WB). GTX83687

GTX83687 WB Image

WB analysis of various cell lines using GTX83687 SAMHD1 antibody [1F6].
Loading : 35 ug per lane

Anti-SAMHD1 antibody [1F6] used in Western Blot (WB). GTX83687

GTX83687 WB Image

WB analysis of various samples using GTX83687 SAMHD1 antibody [1F6].
Loading : 10 ug per lane
Dilution : 1:200

Anti-SAMHD1 antibody [1F6] used in Western Blot (WB). GTX83687

GTX83687 WB Image

The data was published in the journal Retrovirology in 2013. PMID: 24245672

REFERENCE

Application Reference

Monit C et al. Proc Natl Acad Sci U S A 2019; (Epub) Positive selection in dNTPase SAMHD1 throughout mammalian evolution.
Application : WB
Reactivity : Human
Furuya AK et al. PLoS Pathog 2016; 12 (4) : e1005581 Sulforaphane Inhibits HIV Infection of Macrophages through Nrf2.
Application : WB
Reactivity : Human
Sharifi HJ et al. J Virol 2014; Cullin 4A and Cullin4B are Interchangeable for HIV Vpr and Vpx Action Through the CRL4 Ubiquitin Ligase Complex.
Application : WB
Reactivity : Human
Nekorchuk MD et al. Retrovirology 2013; 10 : 138 HIV relies on neddylation for ubiquitin ligase-mediated functions.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519