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SAMHD1 antibody [N2C2], Internal

Anti-SAMHD1 antibody [N2C2], Internal used in Western Blot (WB). GTX103751
Anti-SAMHD1 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103751
Anti-SAMHD1 antibody [N2C2], Internal used in Western Blot (WB). GTX103751
Anti-SAMHD1 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103751

Cat No. GTX103751

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

HeLa , THP-1

Predict Reactivity

Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SAMHD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1 , CHBL2 , DCIP , HDDC1 , MOP-5 , SBBI88 , hSAMHD1

Background

This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SAMHD1 antibody [N2C2], Internal used in Western Blot (WB). GTX103751

GTX103751 WB Image

Wild-type (WT) and SAMHD1 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SAMHD1 antibody [N2C2], Internal (GTX103751) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SAMHD1 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103751

GTX103751 IHC-P Image

SAMHD1 antibody [N2C2], Internal detects SAMHD1 protein at nucleus on SkHep1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SkHep1 xenograft.
SAMHD1 antibody [N2C2], Internal (GTX103751) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SAMHD1 antibody [N2C2], Internal used in Western Blot (WB). GTX103751

GTX103751 WB Image

Sample (30 ug of whole cell lysate)
A: THP-1
7.5% SDS PAGE
GTX103751 diluted at 1:500

Anti-SAMHD1 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103751

GTX103751 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SAMHD1(GTX103751) antibody at 1:500 dilution.

REFERENCE
There are currently no references for SAMHD1 antibody [N2C2], Internal (GTX103751). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SAMHD1 antibody [N2C2], Internal (GTX103751). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf