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SAP102 antibody

Anti-SAP102 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133279
Anti-SAP102 antibody used in Western Blot (WB). GTX133279
Anti-SAP102 antibody used in IHC (Paraffin sections) (IHC-P). GTX133279

Cat No. GTX133279

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all SAP102 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of mouse SAP102. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

discs large MAGUK scaffold protein 3 , Dlgh3 , SAP102 , mKIAA1232

Background

Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling.

Database

Research Area

DATA IMAGES

Anti-SAP102 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133279

GTX133279 ICC/IF Image

SAP102 antibody detects SAP102 protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SAP102 protein stained by SAP102 antibody (GTX133279) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-SAP102 antibody used in Western Blot (WB). GTX133279

GTX133279 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SAP102 antibody (GTX133279) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SAP102 antibody used in IHC (Paraffin sections) (IHC-P). GTX133279

GTX133279 IHC-P Image

SAP102 antibody detects SAP102 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
SAP102 antibody (GTX133279) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169