SAP130 antibody [C3], C-term

Cat. No. GTX106450

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all SF3B3 products

Datasheet
Document

View product citations for antibody GTX106450 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Drosophila, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SAP130. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241320

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

splicing factor 3b subunit 3 , RSE1 , SAP130 , SF3b130 , STAF130

Cellular Localization

Nucleus

Background

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq]

Database

Gene ID: 23450 SF3B3
UniProt: Q15393 SF3B3

Research Area

DATA IMAGES

GTX106450 WB Image

SAP130 antibody [C3], C-term detects SAP130 protein by western blot analysis.
A. 30 μg HeLa whole cell extract
5 % SDS-PAGE
SAP130 antibody [C3], C-term (GTX106450) dilution: 1:10000

GTX106450 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SAP130(GTX106450) antibody at 1:500 dilution.

GTX106450 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Saos-2 xenograft, using SF3B3 (GTX106450) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for SAP130 antibody [C3], C-term (GTX106450). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for SAP130 antibody [C3], C-term (GTX106450). Be the first to share your experience with this product.

Submit a Review

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty