GeneTex
United States (US)

SAP130 antibody [C3], C-term

GTX106450_39694_IFA_19011506_903.jpg
GTX106450_40800_WB_19011506_193.jpg
GTX106450_IHC_19011506_530.jpg

Cat No. GTX106450

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SAP130. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Splicing Factor 3B Subunit 3,Rse1,Sap130,Sf3B130,Staf130,Sf3B3

Cellular Localization

Nucleus

Background

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq]

Database

DATA IMAGES
GTX106450_39694_IFA_19011506_903.jpg

GTX106450 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SAP130(GTX106450) antibody at 1:500 dilution.

GTX106450_40800_WB_19011506_193.jpg

GTX106450 WB Image

SAP130 antibody [C3], C-term detects SAP130 protein by western blot analysis.
A. 30 μg HeLa whole cell extract
5 % SDS-PAGE
SAP130 antibody [C3], C-term (GTX106450) dilution: 1:10000

GTX106450_IHC_19011506_530.jpg

GTX106450 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Saos-2 xenograft, using SF3B3 (GTX106450) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169