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SAP62 antibody [N1C1]

Anti-SAP62 antibody [N1C1] used in Western Blot (WB). GTX115068
Anti-SAP62 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115068
Anti-SAP62 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX115068

Cat No. GTX115068

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SAP62. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619617

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

splicing factor 3a subunit 2 , PRP11 , PRPF11 , SAP62 , SF3a66

Cellular Localization

Nucleus

Background

This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein. [provided by RefSeq]

Database

DATA IMAGES

Anti-SAP62 antibody [N1C1] used in Western Blot (WB). GTX115068

GTX115068 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX115068 diluted at 1:1000

Anti-SAP62 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115068

GTX115068 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SAP62(GTX115068) antibody at 1:500 dilution.

Anti-SAP62 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX115068

GTX115068 IHC-P Image

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using SAP62(GTX115068) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for SAP62 antibody [N1C1] (GTX115068). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SAP62 antibody [N1C1] (GTX115068). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169