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SAP62 antibody [N1C1]

Anti-SAP62 antibody [N1C1] used in Western Blot (WB). GTX115068
Anti-SAP62 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX115068
Anti-SAP62 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115068

Cat. No. GTX115068

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SAP62 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Drosophila, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SAP62. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619617

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

splicing factor 3a subunit 2 , PRP11 , PRPF11 , SAP62 , SF3a66

Cellular Localization

Nucleus

Background

This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SAP62 antibody [N1C1] used in Western Blot (WB). GTX115068

GTX115068 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX115068 diluted at 1:1000

Anti-SAP62 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX115068

GTX115068 IHC-P Image

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using SAP62(GTX115068) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SAP62 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115068

GTX115068 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SAP62(GTX115068) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169