GeneTex
United States (US)

SAP62 antibody [N1C1]

GTX115068_WB_18121410_951.jpg
GTX115068_40247_IFA_18121410_378.jpg
GTX115068_40247_IHC_18121410_162.jpg

Cat No. GTX115068

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SAP62. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Splicing Factor 3A Subunit 2,Prp11,Prpf11,Sap62,Sf3A66,Sf3A2

Cellular Localization

Nucleus

Background

This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein. [provided by RefSeq]

Database

DATA IMAGES
GTX115068_WB_18121410_951.jpg

GTX115068 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX115068 diluted at 1:1000

GTX115068_40247_IFA_18121410_378.jpg

GTX115068 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SAP62(GTX115068) antibody at 1:500 dilution.

GTX115068_40247_IHC_18121410_162.jpg

GTX115068 IHC-P Image

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using SAP62(GTX115068) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169