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SAP97 antibody, Internal

Anti-SAP97 antibody, Internal used in Western Blot (WB). GTX88739

Cat. No. GTX88739

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all SAP97 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88739-PEP).

Product Note

This antibody is expected to recognize both reported isoforms (NP_001091894.1and NP_004078.2).

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NSKTRDKGEIPDD, from the internal region of the protein sequence according to NP_001091894.1; NP_004078.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

discs large MAGUK scaffold protein 1 , DLGH1 , SAP-97 , SAP97 , hdlg

Cellular Localization

Membrane , Endoplasmic reticulum membrane , Cell junction, synapse , Apical cell membrane , Cell junction , Cytoplasm

Background

This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. A multitude of transcript variants deriving from alternative splicing and the use of multiple alternate promoter have been observed, including some splice variants that may be specific to brain and other tissues. An upstream uORF may regulate translation at some splice variants of this gene. [provided by RefSeq, Sep 2018]

Database

Research Area

DATA IMAGES

Anti-SAP97 antibody, Internal used in Western Blot (WB). GTX88739

GTX88739 WB Image

WB analysis of Kelly cell lysate using GTX88739 SAP97 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399