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SAP97 antibody

Anti-SAP97 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23437
Anti-SAP97 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23437
Anti-SAP97 antibody used in Western Blot (WB). GTX23437
Anti-SAP97 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23437
Anti-SAP97 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23437

Cat No. GTX23437

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, IP, IHC

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:5000
ICC/IF 1:250
IHC-Fr 1:1,000
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Product Note

This antibody detects Synapse-Associated Protein 97 (SAP97) from human, rat and mouse tissues. No cross-reactivity to other proteins, including synapse-associated protein family members, has been observed.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide corresponding to residues V(115) L P S E R I S P Q V P N E V L G P E(133) of rat SAP97.

Purification

Ammonium sulfate precipitation

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

discs large MAGUK scaffold protein 1 , B130052P05Rik , Dlgh1 , E-dlg/SAP97 , SAP-97 , SAP97 , mKIAA4187

Cellular Localization

Membrane,Endoplasmic reticulum membrane,Cell junction, synapse,Cell junction,Cytoplasm,Apical cell membrane

Background

Synapse-Associated Protein 97 (SAP97/human homologue of Drosophila discs-large tumor supressor or HDLG) is one of a family of plasma membrane-associated proteins found in synaptic junctions. This is a novel, presynaptic membrane protein homologous to SAP90 and the Drosophila discs-large tumor supressor protein. SAP97 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity. SAP97 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and Shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between SAP97 and other proteins. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP97 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP97 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Database

Research Area

DATA IMAGES

Anti-SAP97 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23437

GTX23437 ICC/IF Image

ICC/IF analysis of RSC-96 cells using GTX23437 SAP97 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

Anti-SAP97 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23437

GTX23437 ICC/IF Image

ICC/IF analysis of C2C12 cells using GTX23437 SAP97 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-SAP97 antibody used in Western Blot (WB). GTX23437

GTX23437 WB Image

WB analysis of 25 ug of C6 (lane 1), PC12 (lane 2) and rat brain (lane 3) cell lysates using GTX23437 SAP97 antibody.
Dilution : 1:5000

Anti-SAP97 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23437

GTX23437 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX23437 SAP97 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-SAP97 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23437

GTX23437 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX23437 SAP97 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339