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SAR1B antibody

Anti-SAR1B antibody used in Western Blot (WB). GTX109170
Anti-SAR1B antibody used in IHC (Paraffin sections) (IHC-P). GTX109170
Anti-SAR1B antibody used in Western Blot (WB). GTX109170
Anti-SAR1B antibody used in IHC (Paraffin sections) (IHC-P). GTX109170
Anti-SAR1B antibody used in Western Blot (WB). GTX109170
Anti-SAR1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109170

Cat. No. GTX109170

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SAR1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Mouse brain , rat brain , HeLa

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human SAR1B Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951807

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secretion associated Ras related GTPase 1B , ANDD , CMRD , GTBPB , SARA2

Cellular Localization

Endoplasmic reticulum membrane; Peripheral membrane protein , Golgi apparatus , Golgi stack membrane; Peripheral membrane protein

Background

The protein encoded by this gene is a small GTPase that acts as a homodimer. The encoded protein is activated by the guanine nucleotide exchange factor PREB and is involved in protein transport from the endoplasmic reticulum to the Golgi. This protein is part of the COPII coat complex. Defects in this gene are a cause of chylomicron retention disease (CMRD), also known as Anderson disease (ANDD). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SAR1B antibody used in Western Blot (WB). GTX109170

GTX109170 WB Image

SAR1B antibody detects SAR1B protein by western blot analysis.
A. 50 μg rat brain lysate/extract
12% SDS-PAGE
SAR1B antibody (GTX109170) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SAR1B antibody used in IHC (Paraffin sections) (IHC-P). GTX109170

GTX109170 IHC-P Image

SAR1B antibody detects SAR1B protein at cytoplasm in mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
SAR1B antibody (GTX109170) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SAR1B antibody used in Western Blot (WB). GTX109170

GTX109170 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX109170 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SAR1B antibody used in IHC (Paraffin sections) (IHC-P). GTX109170

GTX109170 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using GTBPB(GTX109170) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SAR1B antibody used in Western Blot (WB). GTX109170

GTX109170 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX109170 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SAR1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109170

GTX109170 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GTBPB(GTX109170) antibody at 1:200 dilution.

REFERENCE

Application Reference

Zhu R et al. Mol Cell Endocrinol 2019; 493 : 110470 Defective endoplasmic reticulum export causes proinsulin misfolding in pancreatic β cells.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169