PRODUCT
Summary
SARM1 antibody detects NAD(+) hydrolase SARM1, a key factor with a predicted molecular weight of ~79 kDa. SARM1 acts downstream of neuroinflammatory and necroptotic signaling to induce axon degeneration. SARM1 inhibition is being actively studied to prevent axonal degeneration associated with neurodegenerative disorders.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human SARM1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2909943
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sterile alpha and TIR motif containing 1 , MyD88-5 , SAMD2 , SARM
Cellular Localization
Cytoplasm,Cell projection, dendrite,Cell junction, synapse,Mitochondrion
Database
Research Area
DATA IMAGES
![Anti-SARM1 antibody [HL1066] used in Western Blot (WB). GTX636097](/templates/default/js/lightbox/images/loading.gif "Anti-SARM1 antibody [HL1066] used in Western Blot (WB). GTX636097")
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GTX636097 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with SARM1 antibody [HL1066] (GTX636097) diluted at 1:1000 and competitor's antibody (#Highly cited antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636097 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARM1 antibody [HL1066] (GTX636097) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636097 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARM1 antibody [HL1066] (GTX636097) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX636097 ICC/IF Image
SARM1 antibody [HL1066] detects SARM1 protein by immunofluorescent analysis.Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARM1 stained by SARM1 antibody [HL1066] (GTX636097) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636097 IHC-P Image
SARM1 antibody [HL1066] detects SARM1 protein at cytoplasm and mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.SARM1 stained by SARM1 antibody [HL1066] (GTX636097) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for SARM1 antibody [HL1066] (GTX636097). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SARM1 antibody [HL1066] (GTX636097). Be the first to share your experience with this product.
![Anti-SARM1 antibody [HL1066] used in Western Blot (WB). GTX636097](/upload/website/prouct_img/small/GTX636097/GTX636097_T-44298_20210625_WB_M_brain_competitor_watermark_w_23061202_801.webp "Anti-SARM1 antibody [HL1066] used in Western Blot (WB). GTX636097")
![Anti-SARM1 antibody [HL1066] used in Western Blot (WB). GTX636097](/upload/website/prouct_img/small/GTX636097/GTX636097_44340_20210618_WB_M_R_w_23061202_274.webp "Anti-SARM1 antibody [HL1066] used in Western Blot (WB). GTX636097")
![Anti-SARM1 antibody [HL1066] used in Western Blot (WB). GTX636097](/upload/website/prouct_img/small/GTX636097/GTX636097_45145_20231222_WB_TPM_watermark_23122619_558.webp "Anti-SARM1 antibody [HL1066] used in Western Blot (WB). GTX636097")
![Anti-SARM1 antibody [HL1066] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636097](/upload/website/prouct_img/small/GTX636097/GTX636097_44340_20221230_ICC_IF_22122901_850.webp "Anti-SARM1 antibody [HL1066] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636097")
![Anti-SARM1 antibody [HL1066] used in IHC (Paraffin sections) (IHC-P). GTX636097](/upload/website/prouct_img/small/GTX636097/GTX636097_44340_20221230_IHC-P_multiple_M_22122901_941.webp "Anti-SARM1 antibody [HL1066] used in IHC (Paraffin sections) (IHC-P). GTX636097")
![Anti-SARM1 antibody [HL1066] used in Western Blot (WB). GTX636097](/upload/website/prouct_img/normal/GTX636097/GTX636097_T-44298_20210625_WB_M_brain_competitor_watermark_w_23061202_801.webp "Anti-SARM1 antibody [HL1066] used in Western Blot (WB). GTX636097")
![Anti-SARM1 antibody [HL1066] used in Western Blot (WB). GTX636097](/upload/website/prouct_img/normal/GTX636097/GTX636097_44340_20210618_WB_M_R_w_23061202_274.webp "Anti-SARM1 antibody [HL1066] used in Western Blot (WB). GTX636097")
![Anti-SARM1 antibody [HL1066] used in Western Blot (WB). GTX636097](/upload/website/prouct_img/normal/GTX636097/GTX636097_45145_20231222_WB_TPM_watermark_23122619_558.webp "Anti-SARM1 antibody [HL1066] used in Western Blot (WB). GTX636097")
![Anti-SARM1 antibody [HL1066] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636097](/upload/website/prouct_img/normal/GTX636097/GTX636097_44340_20221230_ICC_IF_22122901_850.webp "Anti-SARM1 antibody [HL1066] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636097")
![Anti-SARM1 antibody [HL1066] used in IHC (Paraffin sections) (IHC-P). GTX636097](/upload/website/prouct_img/normal/GTX636097/GTX636097_44340_20221230_IHC-P_multiple_M_22122901_941.webp "Anti-SARM1 antibody [HL1066] used in IHC (Paraffin sections) (IHC-P). GTX636097")