Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARM1 antibody [HL1070] (GTX636102) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARM1 antibody [HL1070] (GTX636102) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARM1 antibody [HL1070] detects SARM1 protein by immunofluorescent analysis.
Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARM1 stained by SARM1 antibody [HL1070] (GTX636102) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).