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SARM1 antibody

Anti-SARM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131411
Anti-SARM1 antibody used in Western Blot (WB). GTX131411
Anti-SARM1 antibody used in Western Blot (WB). GTX131411
Anti-SARM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131411

Cat No. GTX131411

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Drosophila
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SARM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

sterile alpha and TIR motif containing 1,MyD88-5,SAMD2,SARM

Cellular Localization

Cytoplasm

Background

Involved in innate immnune response. Acts as a negative regulator of TICAM1/TRIF-dependent Toll-like receptor signaling by inhibiting induction of TLR3- and TLR4-dependent genes. Specifically blocks TICAM1/TRIF-dependent transcription-factor activation and gene induction, without affecting the MYD88-dependent pathway or non-TLR signaling. Negative regulator of NF-kappa-B and IRF activation.

Database

Research Area

DATA IMAGES
Anti-SARM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131411

GTX131411 ICC/IF Image

SARM1 antibody detects SARM1 protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARM1 protein stained by SARM1 antibody (GTX131411) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARM1 antibody used in Western Blot (WB). GTX131411

GTX131411 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARM1 antibody (GTX131411) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 21555464

Anti-SARM1 antibody used in Western Blot (WB). GTX131411

GTX131411 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARM1 antibody (GTX131411) diluted at 1:500.

Anti-SARM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131411

GTX131411 IHC-P Image

SARM1 antibody detects SARM1 protein at cytoplasm in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
SARM1 antibody (GTX131411) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169
Current in production