APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
1:100-1:1000 |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Note :
Lateral Flow
This antibody has been validated by lateral flow assay based on the customer’s feedback.
Sandwich ELISA
Capture : GTX635686 / GTX635685 / GTX635680 / GTX635689, Detection : GTX635688 or Capture : GTX635688, Detection : GTX635689
Please notice that the detected antibody needs to be conjugated to HRP to function when paired with capture antibody. Please contact us for custom HRP-conjugated antibody.
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Not tested in other applications.
Product Note
This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2 nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.Gold conjugation version is available upon request.This antibody is able to detect multiple SARS-CoV-2 VOCs, including Omicron variant.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
SARS Coronavirus 2
Immunogen
Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888560
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP
Research Area
DATA IMAGES
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GTX635688 ELISA Image
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.
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GTX635688 ELISA Image
Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2 / BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL)Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Please contact us for custom HRP-conjugated antibody.
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GTX635688 ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (4.8*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.EC50 : 0.006 nM
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GTX635688 ICC/IF Image
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX635688 ELISA Image
Sandwich ELISA detection of recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL)Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635686. Please contact us for custom HRP-conjugated antibody.
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GTX635688 ELISA Image
Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.2 / BA.5 variant, His tag (GTX137219-pro) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL)Please notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635688. Please contact us for custom HRP-conjugated antibody.
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GTX635688 ELISA Image
Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.2 / BA.5 variant, His tag (GTX137219-pro) (21.23-0.31 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
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GTX635688 ELISA Image
Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.4 variant, His tag using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL)Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Please contact us for custom HRP-conjugated antibody.
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GTX635688 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635688 WB Image
SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (GTX136779-pro | GTX03400-pro) (0.5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635688 IHC-P (cell pellet) Image
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635688 ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) and SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (wild type) (GTX135592-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
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GTX635688 ELISA Image
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL. Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635680. Please contact us for custom HRP-conjugated antibody.
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GTX635688 WB Image
Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635688 ELISA Image
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL. Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.
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GTX635688 ELISA Image
Sandwich ELISA detection of recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 (COVID-19) (Wild type or B.1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL).
Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Please contact us for custom HRP-conjugated antibody.
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GTX635688 ELISA Image
Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL)Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Please contact us for custom HRP-conjugated antibody.
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GTX635688 ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (GTX136779-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688), and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
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GTX635688 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX635688 ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
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GTX635688 ELISA Image
Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL)Please notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635688. Please contact us for custom HRP-conjugated antibody.
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REFERENCE
There are currently no references for SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455] (GTX635688). Be the first to share your publications with this product.
REVIEW
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