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SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511]

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

Cat. No. GTX635689

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL5511

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Lateral Flow
This antibody has been validated by lateral flow assay based on the customer’s feedback.

Sandwich ELISA
Capture : GTX635689, Detection : GTX635678 / GTX635688 / GTX635808 / GTX635686-01 or Capture : GTX635688, Detection : GTX635689. Please notice that the detected antibody needs to be conjugated to HRP to function when paired with capture antibody. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing. Gold conjugation version is available upon request.This antibody is able to detect multiple SARS-CoV-2 VOCs, including Omicron variant.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888561

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2 / BA.5 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689

GTX635689 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as detection antibody at concentration of 1 μg/mL.
Sensitivity : 39.06 pg/mL

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689

GTX635689 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / omicron variant, His tag (1 μg, GTX136779-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (4.8*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.014 nM

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635689

GTX635689 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689

GTX635689 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL)
Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL)
Please notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635688. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635689

GTX635689 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) and SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (wild type) (GTX135592-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135357-pro) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689

GTX635689 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL)
Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (GTX136779-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688), and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 (COVID-19) (Wild type or B.1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL).

Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in Western Blot (WB). GTX635689

GTX635689 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.2 / BA.5 variant, His tag (GTX137219-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL)
Please notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635688. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] used in ELISA (ELISA). GTX635689

GTX635689 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.2 / BA.5 variant, His tag (GTX137219-pro) (21.23-0.31 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations