*Optimal dilutions/concentrations should be determined by the researcher.
Application
Recommended Dilution
WB
1:1000-1:10000
ICC/IF
1:100-1:1000
IHC-Fr
Assay dependent
ELISA
Assay dependent
Lateral Flow
Assay dependent
Sandwich ELISA
Assay dependent
IHC-P (cell pellet)
Assay dependent
Note :
Lateral Flow
This antibody has been validated by lateral flow assay based on the customer’s feedback.
Sandwich ELISA
Capture : GTX635689, Detection : GTX635678 / GTX635688 / GTX635808 / GTX635686-01 or Capture : GTX635688, Detection : GTX635689.
Please notice that the detected antibody needs to be conjugated to HRP to function when paired with capture antibody. Please contact us for custom HRP-conjugated antibody.
Not tested in other applications.
Product Note
This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing. Gold conjugation version is available upon request.This antibody is able to detect multiple SARS-CoV-2 VOCs, including Omicron variant.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
SARS Coronavirus 2
Immunogen
Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888561
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2 / BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)
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GTX635689 ELISA Image
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.
Show More +
GTX635689 ELISA Image
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.
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GTX635689 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX635689 ELISA Image
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [GT113] (GTX635808) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as detection antibody at concentration of 1 μg/mL.Sensitivity : 39.06 pg/mL
Show More +
GTX635689 WB Image
SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / omicron variant, His tag (1 μg, GTX136779-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Show More +
GTX635689 ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (4.8*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.EC50 : 0.014 nM
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GTX635689 ICC/IF Image
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
Show More +
GTX635689 ELISA Image
Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)
Show More +
GTX635689 WB Image
SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Show More +
GTX635689 ELISA Image
Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL)Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.
Show More +
GTX635689 ELISA Image
Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL)Please notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635688. Please contact us for custom HRP-conjugated antibody.
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GTX635689 IHC-P (cell pellet) Image
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635689 ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) and SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (wild type) (GTX135592-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Show More +
GTX635689 ELISA Image
Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135357-pro) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)
Show More +
GTX635689 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Show More +
GTX635689 ELISA Image
Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL)Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.
Show More +
GTX635689 ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (GTX136779-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688), and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Show More +
GTX635689 ELISA Image
Sandwich ELISA detection of recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 (COVID-19) (Wild type or B.1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL).
Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.
Show More +
GTX635689 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Show More +
GTX635689 ELISA Image
Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.2 / BA.5 variant, His tag (GTX137219-pro) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL)Please notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635688. Please contact us for custom HRP-conjugated antibody.
Show More +
GTX635689 ELISA Image
Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.2 / BA.5 variant, His tag (GTX137219-pro) (21.23-0.31 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
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