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SARS-CoV-2 (COVID-19) Nucleocapsid antibody

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in ELISA (ELISA). GTX135361
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in Western Blot (WB). GTX135361
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in Western Blot (WB). GTX135361
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135361
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in ELISA (ELISA). GTX135361
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in ELISA (ELISA). GTX135361

Cat. No. GTX135361

Reference 17

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

 SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) nucleocapsid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX135361, Detection: GTX632269
Not tested in other applications.

Product Note

This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) nucleocapsid (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887484

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in ELISA (ELISA). GTX135361

GTX135361 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135361 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in Western Blot (WB). GTX135361

GTX135361 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135361) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in Western Blot (WB). GTX135361

GTX135361 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135361) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135361

GTX135361 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135361) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in ELISA (ELISA). GTX135361

GTX135361 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135361) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in ELISA (ELISA). GTX135361

GTX135361 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135361) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

Application Reference

de Melo GD et al. Nat Commun 2023; 14 (1) : 4485 Neuroinvasion and anosmia are independent phenomena upon infection with SARS-CoV-2 and its variants.
Yang J et al. Nat Microbiol 2023; 8 (1) : 121-134 Fluorogenic reporter enables identification of compounds that inhibit SARS-CoV-2.
Margaroli C et al. Res Sq 2023; Type I interferon-dependent IFIT3 signaling is critical for viral clearance in airway neutrophils.
Margaroli C et al. Front Med (Lausanne) 2023; 10 : 1118024 Spatial transcriptomic profiling of coronary endothelial cells in SARS-CoV-2 myocarditis.
Application : IHC-P
Wu CT et al. Cell 2023; 186 (1) : 112-130.e20 SARS-CoV-2 replication in airway epithelia requires motile cilia and microvillar reprogramming.
Application : IHC-Fr
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REVIEW

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Application Reference
de Melo GD et al. Nat Commun 2023; 14 (1):4485 Neuroinvasion and anosmia are independent phenomena upon infection with SARS-CoV-2 and its variants.
Yang J et al. Nat Microbiol 2023; 8 (1):121-134 Fluorogenic reporter enables identification of compounds that inhibit SARS-CoV-2.
Margaroli C et al. Res Sq 2023; Type I interferon-dependent IFIT3 signaling is critical for viral clearance in airway neutrophils.
Margaroli C et al. Front Med (Lausanne) 2023; 10:1118024 Spatial transcriptomic profiling of coronary endothelial cells in SARS-CoV-2 myocarditis.
Application : IHC-P
Wu CT et al. Cell 2023; 186 (1):112-130.e20 SARS-CoV-2 replication in airway epithelia requires motile cilia and microvillar reprogramming.
Application : IHC-Fr
Niemeyer D et al. PLoS Biol 2022; 20 (11):e3001871 SARS-CoV-2 variant Alpha has a spike-dependent replication advantage over the ancestral B.1 strain in human cells with low ACE2 expression.
Besselink G et al. Biosensors (Basel) 2022; 12 (8) Asymmetric Mach-Zehnder Interferometric Biosensing for Quantitative and Sensitive Multiplex Detection of Anti-SARS-CoV-2 Antibodies in Human Plasma.
Carlotta Pipolo et al. PLoS One 2022; 17 (4):e0266740 Evidence of SARS-CoV-2 in nasal brushings and olfactory mucosa biopsies of COVID-19 patients.
Alison E Burkett et al. Front Med (Lausanne) 2022; 9:811546 Gastrointestinal Manifestations of COVID-19 Infection: Clinicopathologic Findings in Intestinal Resections Performed at Single Institution.
Application : IHC-P
Dorman LC et al. bioRxiv 2022; A type I interferon response defines a conserved microglial state required for effective neuronal phagocytosis.
Application : ICC/IF
Kaufmann E et al. Cell Rep 2022; 38 (10):110502 BCG vaccination provides protection against IAV but not SARS-CoV-2.
Application : IHC-P
Kung YA et al. mBio 2022;:e0271721 Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis.
Application : ICC/IF
Charlotte Steenblock et al. Nat Commun 2021; 12 (1):3534 Viral infiltration of pancreatic islets in patients with COVID-19.
Application : IHC-P
Xiao Y et al. Cell 2021; 184 (25):6037-6051.e14 A defective viral genome strategy elicits broad protective immunity against respiratory viruses.
Application : IHC-P
Wu CT et al. Cell Metab 2021; 33 (8):1565-1576.e5 SARS-CoV-2 infects human pancreatic β cells and elicits β cell impairment.
Application : WB
Margaroli C et al. Cell Rep Med 2021; 2 (4):100242 Spatial mapping of SARS-CoV-2 and H1N1 lung injury identifies differential transcriptional signatures.
Application : IHC-P
Toto V et al. Arch Pathol Lab Med 2021; (Epub) Finding of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) within Placental Tissue 11 Weeks after Maternal Infection.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169