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SARS-CoV-2 (COVID-19) Nucleocapsid antibody

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in Western Blot (WB). GTX135570
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135570
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in Western Blot (WB). GTX135570

Cat. No. GTX135570

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P (cell pellet)

Reactivity

 SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) nucleocapsid products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Product Note

This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2 nucleocapsid proteins. Our internal testing indicates minor cross-reactivity with MERS-CoV nucleocapsid protein.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of SARS-CoV-2 (COVID-19) nucleocapsid (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887498

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in Western Blot (WB). GTX135570

GTX135570 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX135570) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135570

GTX135570 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Nucleocapsid antibody detects SARS-CoV-2 (COVID-19) Nucleocapsid protein at cytoplasm by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) Nucleocapsid stained by SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX135570) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in Western Blot (WB). GTX135570

GTX135570 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135570) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Suzuki R et al. Nature 2022; (Epub) Attenuated fusogenicity and pathogenicity of SARS-CoV-2 Omicron variant.
Application : ICC/IF
Saito A et al. Nature 2021; Enhanced fusogenicity and pathogenicity of SARS-CoV-2 Delta P681R mutation.
Application : ICC/IF
Submit a Reference

REVIEW

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Package List Price ($)
$ 399
$ 169