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SARS-CoV-2 (COVID-19) Nucleocapsid overexpression 293T whole cell lysate

SARS-CoV-2 (COVID-19) Nucleocapsid overexpression 293T whole cell lysate. GTX535665
SARS-CoV-2 (COVID-19) Nucleocapsid overexpression 293T whole cell lysate. GTX535665
SARS-CoV-2 (COVID-19) Nucleocapsid overexpression 293T whole cell lysate. GTX535665

Cat No. GTX535665

Application

WB, ELISA

Species

SARS Coronavirus 2
Package
200 μg ($299)
PRODUCT

Summary

Whole-cell lysate of 293T cells overexpressing SARS-CoV-2 nucleocapsid protein (full-length, N-terminal 3x Flag-tagged). The translated amino acid sequence is based on QHD43423.2. Lysate prepared in RIPA buffer with no reducing agents and is unboiled. Please see matched control 293T lysate (GTX535673)

PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 μg/ml Leupeptin, 2 μg/ml Aprotinin, 1 μg/ml Pepstatin A, 0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Nucleocapsid overexpression 293T whole cell lysate. GTX535665

GTX535665 WB Image

Non-transfected and transfected 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Nucleocapsid overexpression 293T whole cell lysate. GTX535665

GTX535665 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid 293T overexpression cell lysates (GTX535665) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Nucleocapsid overexpression 293T whole cell lysate. GTX535665

GTX535665 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 nucleocapsid (full length) transfected (GTX535665) 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Proclin.pdf