Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] (GTX637321) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] detects SARS-CoV-2 (COVID-19) ORF3a protein by immunohistochemical analysis.
Sample: Paraffin-embedded mock and SARS-CoV-2 (COVID-19) ORF3a transfected 293T.
Green: SARS-CoV-2 (COVID-19) ORF3a stained by SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] (GTX637321) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] detects SARS-CoV-2 (COVID-19) ORF3a protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) ORF3a stained by SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] (GTX637321) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).