SARS-CoV-2 (COVID-19) ORF7b antibody [HL1453]

Cat. No. GTX636925

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1453
Isotype	IgG
Application	WB, ICC/IF, IHC-P (cell pellet)
Reactivity	SARS Coronavirus 2
Package	100 μl ($399), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) ORF7b products

Datasheet

View product citations for antibody GTX636925 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P (cell pellet)	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) ORF7b. (Strain:Wuhan-hu-1)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX636925 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) ORF7b antibody [HL1453] (GTX636925) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636925 ICC/IF Image

SARS-CoV-2 (COVID-19) ORF7b antibody [HL1453] detects SARS-CoV-2 (COVID-19) ORF7b protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) ORF7b stained by SARS-CoV-2 (COVID-19) ORF7b antibody [HL1453] (GTX636925) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636925 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) ORF7b antibody [HL1453] detects SARS-CoV-2 (COVID-19) ORF7b protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and SARS-CoV-2 ORF7b transfected 293T cell pellet.
Green: SARS-CoV-2 (COVID-19) ORF7b stained by SARS-CoV-2 (COVID-19) ORF7b antibody [HL1453] (GTX636925) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

SARS-CoV-2 (COVID-19) ORF7b antibody [HL1453]

Cat. No. GTX636925

Host

Clonality

Clone Name

Isotype

Application

Reactivity