SARS-CoV-2 (COVID-19) ORF8 antibody

Cat. No. GTX135591

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, ELISA, IHC-P (cell pellet)
Reactivity	SARS Coronavirus 2
Package	100 μl ($399), 25 μl ($169)

See all SARS-CoV-2 (COVID-19) ORF8 products

Datasheet
Reference

View product citations for antibody GTX135591 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent
ELISA	Assay dependent
IHC-P (cell pellet)	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) ORF8 (Strain:Wuhan-Hu-1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887502

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

GTX135591 ICC/IF Image

SARS-CoV-2 (COVID-19) ORF8 antibody detects SARS-CoV-2 (COVID-19) ORF8 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) ORF8 stained by SARS-CoV-2 (COVID-19) ORF8 antibody (GTX135591) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX135591 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) ORF8 antibody detects SARS-CoV-2 (COVID-19) ORF8 protein at cytoplasm by immunohistochemical analysis.
Sample: Mock and SARS-CoV-2 (COVID-19) ORF8 transfected 293T cell FFPE Cell Pellet Block
Green: SARS-CoV-2 (COVID-19) ORF8 stained by SARS-CoV-2 (COVID-19) ORF8 antibody (GTX135591) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX135591 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 ORF8 antibody (GTX135591) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.