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SARS-CoV-2 (COVID-19) nsp12 antibody

Anti-SARS-CoV-2 (COVID-19) nsp12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135469
Anti-SARS-CoV-2 (COVID-19) nsp12 antibody used in Western Blot (WB). GTX135469

Cat. No. GTX135469

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) nsp12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Product Note

This antibody was raised against N-terminal domain of nsp12 protein, and it does not react with RdRp enzyme domain.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) nsp12 (Strain:Wuhan-Hu-1). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2887497

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV RdRp , SARS-CoV-2 RdRp , COVID-19 RdRp , 2019-nCoV RNA-directed RNA polymerase , SARS-CoV-2 RNA-directed RNA polymerase , COVID-19 RNA-directed RNA polymerase , 2019-nCoV nsp12 , SARS-CoV-2 nsp12 , COVID-19 nsp12

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nsp12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135469

GTX135469 ICC/IF Image

SARS-CoV-2 (COVID-19) nsp12 antibody detects SARS-CoV-2 (COVID-19) nsp12 protein by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp12 stained by SARS-CoV-2 (COVID-19) nsp12 antibody (GTX135469) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) nsp12 antibody used in Western Blot (WB). GTX135469

GTX135469 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp12 antibody (GTX135469) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169