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SARS-CoV-2 (COVID-19) Spike (D614G Mutant) protein (ECD), His tag (active)

SARS-CoV-2 (COVID-19) Spike (D614G Mutant) protein (ECD), His tag (active). GTX02575-pro
SARS-CoV-2 (COVID-19) Spike (D614G Mutant) protein (ECD), His tag (active). GTX02575-pro
SARS-CoV-2 (COVID-19) Spike (D614G Mutant) protein (ECD), His tag (active). GTX02575-pro
SARS-CoV-2 (COVID-19) Spike (D614G Mutant) protein (ECD), His tag (active). GTX02575-pro
SARS-CoV-2 (COVID-19) Spike (D614G Mutant) protein (ECD), His tag (active). GTX02575-pro
SARS-CoV-2 (COVID-19) Spike (D614G Mutant) protein (ECD), His tag (active). GTX02575-pro

Cat No. GTX02575-pro

Application

ELISA, Functional Assay

Species

SARS Coronavirus 2
Package
100 μg ($499)

APPLICATION

Observed MW

190 kDa.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

C-terminal 8x His tagged; 16-1213 a.a. (Uniprot #P0DTC2, D614G variant). Full-length soluble with foldon trimerization motif, mutated Furin recognition site and 3 stabilising mutations (F817P, K986P and V987P), based on/modified from Hsieh et al, 2020.

Expression System

HEK293 cells

Purification

Purified by affinity chromatography

Purity

> 90% by SDS-PAGE

Endotoxin

< 0.1 ng/μg (1 IEU/μg) determined by LAL method

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

spike glycoprotein

Target Info

The original Wuhan strain of SARS CoV2 virus has become quickly replaced by its more transmissible variant, mainly determined by a single amino acid point mutation D614G of Spike protein.

Database

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike (D614G Mutant) protein (ECD), His tag (active). GTX02575-pro

GTX02575-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (D614G variant) protein, His tag (active) (GTX02575-pro), and SARS-CoV-2 spike (trimer) protein (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (13111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (D614G Mutant) protein (ECD), His tag (active). GTX02575-pro

GTX02575-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (D614G variant) protein, His tag (active) (GTX02575-pro), and SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) Spike (D614G Mutant) protein (ECD), His tag (active). GTX02575-pro

GTX02575-pro Image

CD spectra of different cation forms of spike protein. The scanning fluorimetry data of the two batches shows the protein is both stable and functional, with heparin binding activity.
A and C : CD spectra were recorded on J-1100 spectrometer between 180 and 260 nm of spike protein (0.5 mg/ml).
B and D : Secondary structure were analysed by program CDSSTR of A and C, respectively.
A and B : the first batch of spike protein (PBS buffer).
C and D : the second batch of spike protein (PBS, 20% glycerol).

SARS-CoV-2 (COVID-19) Spike (D614G Mutant) protein (ECD), His tag (active). GTX02575-pro

GTX02575-pro Image

Gel filtration analysis of 2 different batches of spike protein.
The first batch : spike protein in PBS buffer.
The second batch : spike protein in PBS, 20% glycerol.

SARS-CoV-2 (COVID-19) Spike (D614G Mutant) protein (ECD), His tag (active). GTX02575-pro

GTX02575-pro Image

Differential scanning fluorimetry (DFS) analysis the stability and the heparin binding ability. DSF of proteins were performed in the absence or presence of heparin (10 μM). The scanning fluorimetry data of the two batches shows the protein is both stable and functional, with heparin binding activity.
A and C : Melting curve of spike protein.
B and D : First derivative of the melting curves of spike to show its melting temperature as peak.
A and B : the first batch of spike protein (PBS)
C and D : the second batch of spike protein (PBS, 20% glycerol).

SARS-CoV-2 (COVID-19) Spike (D614G Mutant) protein (ECD), His tag (active). GTX02575-pro

GTX02575-pro Image

SDS-PAGE of GTX02575-pro SARS-CoV-2 (COVID-19) Spike (D614G variant) protein, His tag.

REFERENCE

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REVIEW

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