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SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136059-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136059-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136059-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136059-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136059-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136059-pro

Cat No. GTX136059-pro

Application

Functional Assay

Species

SARS Coronavirus 2
Package
100 μg ($499)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
Functional Assay Assay dependent
Not tested in other applications.

Product Note

These mutations (deletion 69-70, deletion 144, N501Y, A570D, D614G, P681H,T716I, S982A, D1118H) are found in the SARS-CoV-2 UK variant (501Y.V1 / B.1.1.7).

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike of QHD43416.1 (1-1213 a.a, extracellular domain) with UK variant (501Y.V1 / B.1.1.7) mutations (deletion 69-70, deletion 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H ), 682-685GSAS mutations, 6 stabilizing (HexaPro) mutations (F817P, A892P, A899P, A942P, K986P,V987P), and His tag at the C-terminus.

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SARS-CoV-2 alpha , SARS2 alpha , B.1.1.7 , 501Y.v1

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136059-pro

GTX136059-pro Image

SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (GTX136059-pro, 0.25 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136059-pro

GTX136059-pro Image

SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (GTX136059-pro, 0.25 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136059-pro

GTX136059-pro Image

1 μg of GTX136059-pro SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136059-pro

GTX136059-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (active) (UK variant) (GTX136059-pro), SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike Protein (Wild type) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (14-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136059-pro

GTX136059-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (active) (UK variant) (GTX136059-pro), SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike Protein (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136059-pro

GTX136059-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (active) (UK variant) (GTX136059-pro), SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike Protein (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

REFERENCE

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REVIEW

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