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SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro

Cat No. GTX136061-pro

Application

ELISA, Functional Assay

Species

SARS Coronavirus 2
Package
100 μg ($499)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ELISA Assay dependent
Functional Assay Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike of QHD43416.1 (1-1213 a.a, extracellular domain) with South Africa variant (501Y.V2 / B.1.351) mutations (L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G, A701V), S1-S2 cleavage site mutations (682-685GSAS), 6 stabilizing (HexaPro) mutations (F817P, A892P, A899P, A942P, K986P,V987P), and His tag at the C-terminus.

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SARS-CoV-2 beta , SARS2 beta , B.1.351 , 501Y.v2

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro

GTX136061-pro Image

0.5 μg of GTX136061-pro SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro

GTX136061-pro Image

SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (GTX136061-pro, 0.2 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro

GTX136061-pro Image

SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (GTX136061-pro, 0.2 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:150000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro

GTX136061-pro Image

SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (GTX136061-pro, 0.2 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 / S2' antibody [HL1039] (GTX635911) diluted at 1:140000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro

GTX136061-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (active) (UK variant) (GTX136059-pro), SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike Protein (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro

GTX136061-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (active) (UK variant) (GTX136059-pro), SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike Protein (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro

GTX136061-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro

GTX136061-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro

GTX136061-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Mouse IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro

GTX136061-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro

GTX136061-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro

GTX136061-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.351 / Beta variant, His tag (active). GTX136061-pro

GTX136061-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (active) (UK variant) (GTX136059-pro), SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike Protein (Wild type) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (14-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

REFERENCE

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