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SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

Cat No. GTX136432-pro

Application

ELISA, Functional Assay, Sandwich ELISA

Species

SARS Coronavirus 2
Package
100 μg ($499)

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA:
Capture: GTX635807, Detection: GTX635791.
Please notice that GTX635791 needs to be conjugated to HRP when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike of QHD43416.1 (1-1213 a.a, extracellular domain) with delta variant (B.1.617.2) mutations (T19R, G142D, del156-157, R158G, L452R, T478K, D614G, P681R, D950N), S1-S2 cleavage site mutations (682-685GSAS), 6 stabilizing (HexaPro) mutations (F817P, A892P, A899P, A942P, K986P,V987P), and T4 trimerization domain as well as a C-terminal His tag.

Expression System

HEK293 cells

Purity

>95%

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Delta variant Spike , B.1.617.2 Spike , SARS-CoV-2 Delta variant Spike , COVID-19 Delta variant Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL)
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.03-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (1 μg/mL). Human IgG antibody (HRP) (1:200000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 499