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SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

Cat No. GTX136432-pro

Application

ELISA, Functional Assay, Sandwich ELISA

Species

SARS Coronavirus 2
Package
100 μg ($499)

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA:
Capture: GTX635807, Detection: GTX635791.
Please notice that GTX635791 needs to be conjugated to HRP when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS buffer. Reconstitute with distilled water.

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution and aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Region/Sequence

SARS-CoV-2 Spike of QHD43416.1 (1-1213 a.a, extracellular domain) with delta variant (B.1.617.2) mutations (T19R, G142D, del156-157, R158G, L452R, T478K, D614G, P681R, D950N), S1-S2 cleavage site mutations (682-685GSAS), 6 stabilizing (HexaPro) mutations (F817P, A892P, A899P, A942P, K986P,V987P), and His tag at the C-terminus.

Expression System

HEK293 cells

Purity

>95%

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SARS-CoV-2 dleta , SARS2 delta , B.1.617.2 , Delta variant

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL)
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (1 μg/mL). Human IgG antibody (HRP) (1:200000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.03-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.617.2 / delta variant, His tag (active). GTX136432-pro

GTX136432-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 499