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SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag. GTX137037-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag. GTX137037-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag. GTX137037-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag. GTX137037-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag. GTX137037-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag. GTX137037-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag. GTX137037-pro

Cat No. GTX137037-pro

Application

Binding Assay, WB, ELISA

Species

SARS Coronavirus 2
Package
100 μg ($499)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

0.28 mg/ml

Region/Sequence

SARS-CoV-2 Spike of QHD43416.1 (1-1213 a.a, extracellular domain) with Omicron variant (BA.1 / B.1.1.529) mutations (T19I, L24del, P25del, P26del, A27S, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K), and T4 trimerization domain as well as a C-terminal His tag.

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Omicron variant Spike , BA.2 Spike , BA.5 Spike , SARS-CoV-2 Omicron variant Spike , COVID-19 Omicron variant Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag. GTX137037-pro

GTX137037-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, BA.2 / Omicron variant, His tag (GTX137037-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag. GTX137037-pro

GTX137037-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant; BA.2 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag. GTX137037-pro

GTX137037-pro ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, BA.2 / Omicron variant, His tag (GTX137037-pro) (29.71-0.46 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag. GTX137037-pro

GTX137037-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag. GTX137037-pro

GTX137037-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag. GTX137037-pro

GTX137037-pro WB Image

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, BA.2 / Omicron variant, His tag (1 μg, GTX137037-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2 variant, His tag. GTX137037-pro

GTX137037-pro Image

GTX137037-pro SARS-CoV-2 (COVID-19) Spike (ECD) Protein, BA.2 / Omicron variant, His tag protein were analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

REFERENCE

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REVIEW

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Package List Price ($)
$ 499