GeneTex

  • Country / Location Selection

United States (US)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag. GTX137881-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag. GTX137881-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag. GTX137881-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag. GTX137881-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag. GTX137881-pro

Cat. No. GTX137881-pro

Application

 Binding Assay, ELISA, Sandwich ELISA

Species

 SARS Coronavirus 2
Package
100 μg ($499)
See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA: Capture: GTX635807, Detection: GTX635793-01

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with distilled water to 0.2 mg/ml. Lyophilized from PBS, 2% Trehalose, 5% Mannitol, 0.01% Tween-80.

Preservative

No Preservative

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Region/Sequence

SARS-CoV-2 Spike of QHD43416.1 (1-1213 a.a, extracellular domain) with Omicron variant (BF.7) mutations (T19I, L24del, P25del, P26del, A27S, H69del, V70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, K444T, L452R, N460K, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K, HexaPro (F817P, A892P, A899P, A942P, K986P, and V987P) and T4 trimerization domain as well as a C-terminal His tag.

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag. GTX137881-pro

GTX137881-pro ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (GTX137881-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag. GTX137881-pro

GTX137881-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag. GTX137881-pro

GTX137881-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.4/ BA.5 Omicron variant; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag. GTX137881-pro

GTX137881-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (GTX137881-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1867] (GTX637592) (1 μg/mL)
Please notice that GTX637592 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag. GTX137881-pro

GTX137881-pro Image

GTX137881-pro SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron BQ.1 variant, His tag was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

REFERENCE

There are currently no references for SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (GTX137881-pro). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (GTX137881-pro). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 499