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SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

Cat No. GTX136091-pro

Application

ELISA, Functional Assay, Sandwich ELISA

Species

SARS Coronavirus 2
Package
100 μg ($499)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike of QHD43416.1 (1-1213 a.a, extracellular domain) with Brazil variant (P.1 / 501Y.V3 / B.1.1.28) mutations (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F), S1-S2 cleavage site mutations (682-685GSAS), 6 stabilizing (HexaPro) mutations (F817P, A892P, A899P, A942P, K986P,V987P), and His tag at the C-terminus.

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SARS-CoV-2 gamma , SARS2 gamma , P.1 , 501Y.v3

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL)
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro Image

SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (0.2 μg, GTX136091-pro) were separated by 12% SDS-PAGE, and the membrane were blotted with various antibodies diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro Image

1 μg of GTX136091-pro SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (GTX136091-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13.72-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

REFERENCE

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REVIEW

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