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SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active)

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro
SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

Cat. No. GTX135972-pro

Application

Binding Assay, WB, ELISA, Sandwich ELISA

Species

SARS Coronavirus 2
Package
100 μg ($499)

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA:
Capture: GTX635807, Detection: GTX635792-01 / GTX635791. or >Capture: GTX635693, Detection: GTX636042.

Calculated MW

133 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike of QHD43416.1 (1-1213 a.a, extracellular domain ) with S1-S2 cleavage site mutations (682-685GSAS), 6 stabilizing mutations ( HexaPro mutations, including F817P, A892P, A899P, A942P, K986P, and V987P) and T4 trimerization domain as well as a C-terminal His tag.

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SARS-CoV-2 Spike , COVID-19 Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL)
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2.12.1 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2.12.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro Image

GTX135972-pro SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) protein were analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.03-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Mouse IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro WB Image

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (0.5 μg, GTX135972-pro) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (1 μg/mL). Human IgG antibody (HRP) (1:200000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active). GTX135972-pro

GTX135972-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

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$ 499