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SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

Cat. No. GTX136091-pro

Application

 Binding Assay, WB, ELISA, Sandwich ELISA

Species

 SARS Coronavirus 2
Package
100 μg ($499)
See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA:
Capture : GTX635807, Detection : GTX635791 / GTX635793-01 / GTX635792-01 or Capture : GTX632604, Detection : GTX635792 / GTX635672 / GTX635713.
Please notice that the detected antibody needs to be conjugated to HRP to function when paired with capture antibodies. Please contact us for custom HRP-conjugated antibody.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike of QHD43416.1 (1-1213 a.a, extracellular domain) with Brazil variant (P.1 / 501Y.V3 / B.1.1.28) mutations (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F), S1-S2 cleavage site mutations (682-685GSAS), 6 stabilizing (HexaPro) mutations (F817P, A892P, A899P, A942P, K986P,V987P), and T4 trimerization domain as well as a C-terminal His tag.

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Gamma variant Spike , P.1 Spike , SARS-CoV-2 Gamma variant Spike , COVID-19 Gamma variant Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL)
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL)

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro Image

1 μg of GTX136091-pro SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant (GTX136091-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.15-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active) (GTX136091-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro WB Image

SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (0.2 μg, GTX136091-pro) were separated by 12% SDS-PAGE, and the membrane were blotted with various antibodies diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active). GTX136091-pro

GTX136091-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active) (GTX136091-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

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Package List Price ($)
$ 499