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SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active)

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

Cat. No. GTX136022-pro

Reference 1

Application

 Binding Assay, WB, ELISA, Sandwich ELISA

Species

 SARS Coronavirus 2
Package
100 μg ($499)
See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

Recommended antibody pair for sandwich ELISA:
Capture: GTX635807, Detection: GTX635792-01 / GTX635793-01

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a) with K417N, E484K, and N501Y mutations and His tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Beta variant Spike , B.1.351 Spike , SARS-CoV-2 Beta variant Spike , COVID-19 Beta variant Spike

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro WB Image

SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (GTX136022-pro, 1 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro Binding Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro Binding Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (13111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro Image

1μg of GTX136022-pro SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1000-1.19*10-4 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 1.30 μM

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1000-1.19*10-4 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 0.43 μM

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro Binding Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro Binding Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (UK variant) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (South Africa variant) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (Brazil variant) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (UK variant) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.351 / Beta variant, His tag (active). GTX136022-pro

GTX136022-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) as detection antibody at concentration of 1 μg/mL.

REFERENCE

Application Reference

TW Liu et al. J Ethnopharmacol 2024; 320 : 117412 Polymethoxyflavone from Citrus depressa as an inhibitor against various variants of SARS-CoV-2 spike protein
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Package List Price ($)
$ 499