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SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active)

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro
SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro

Cat No. GTX136014-pro

Application

ELISA, Functional Assay, Sandwich ELISA

Species

SARS Coronavirus 2
Package
100 μg ($499)

APPLICATION

Application Note

Recommended antibody pair for sandwich ELISA:
Capture: GTX635807, Detection: GTX635792-01 / GTX635793-01

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS buffer. Reconstitute with distilled water.

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution and aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Region/Sequence

SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a) with N501Y mutation and His tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Alpha variant , B.1.1.7 , 501Y.v1 , SARS-CoV-2 alpha , SARS2 alpha

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro

GTX136014-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro

GTX136014-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro

GTX136014-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro

GTX136014-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro

GTX136014-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (13111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro

GTX136014-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) and SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro

GTX136014-pro Image

0.5μg of GTX136014-pro SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro

GTX136014-pro Image

SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro, 0.2 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro

GTX136014-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (UK variant) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (South Africa variant) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (Brazil variant) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (UK variant) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro

GTX136014-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (UK variant) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (South Africa variant) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (Brazil variant) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (UK variant) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.1.7 / Alpha variant, His tag (active). GTX136014-pro

GTX136014-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (UK variant) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (South Africa variant) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (Brazil variant) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (UK variant) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

REFERENCE

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REVIEW

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